PPS073

antibody from R&D Systems

Targeting: GABBR2 GABABR2, GPR51, GPRC3B, HG20

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

PPS073

Provider

R&D Systems

Product name

Phospho-GABA-B R2 (S892) Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Human, mouse, rat, and Xenopus ~110 kDa GABAB Receptor, R2 subunit phosphorylated at S892

Reactivity

Human, Mouse, Rat, Xenopus

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 uL

Storage

For long-term storage,

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Phospho-GABA_B R2 (S892) by Western Blot Western blots of COS-7 cells transiently expressing mutant GABA_B R2 (S892A) (lane 1), wild type GABA_B R2 (lane 2) or control untransfected cells (lane 3) were lysed and 50 mg of protein from each sample was separated by SDS-PAGE and transferred to a nitrocellulose membrane. Blots were then immunolabeled with rabbit anti-phospho-GABA_B R2 (S892) (A). In B, the membrane was pre-treated with lambda-PPase (1200 units for 30 minutes) before being exposed to the anti-GABA_B Receptor, R2 subunit (S892). The immunolabeling is completely eliminated by treatment with lambda-PPase.