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antibody from Invitrogen Antibodies
Targeting: CPSF6
CFIM, CFIM68, CFIM72, HPBRII-4, HPBRII-7
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-117890 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CPSF6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of CPSF6 in Lane A: Jurkat Whole Cell lysate, Lane B: K562 Whole Cell lysate, Lane C: A431 Whole Cell lysate, Lane D: HepG2 Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117890) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG H&L (Dylight 800) using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 59 kDa, Observed band size: 72 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CPSF6 Polyclonal Antibody (Product # PA5-117890) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: K562 Whole Cell Lysate, Lane C: A431 Whole Cell Lysate, Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 59 kDa. Observed band size: 72 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of CPSF6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CPSF6 Polyclonal Antibody (Product # PA5-117890, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CPSF6 in human breast carcinoma with CPSF6 Polyclonal Antibody (Product # PA5-117890, 1:1,000, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human CPSF6 in human colon carcinoma with CPSF6 Polyclonal Antibody (Product # PA5-117890, 1:1,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CPSF6 in Lane A: 0.5 mg Hela Whole Cell lysate, Lane B: 0.5 mg K562 Whole Cell lysate, Lane C: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117890) with a dilution of 1:100 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 60 kDa, Observed band size: 72 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CPSF6 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg K562 Whole Cell Lysate, Lane C: 0.5 mg Jurkat Whole Cell Lysate 4 µL with CPSF6 Polyclonal Antibody (Product # PA5-117890) and 15 µL of 50 % Protein G agarose. Primary antibody: CPSF6 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 72 kDa.
