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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- R32489 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ABHD5 Antibody / CGI-58
- Antibody type
- Polyclonal (Antigen purified)
- Description
- Antigen affinity purified
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ABHD5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat kidney and 2) human A431 lysate with ABHD5 antibody at 0.5ug/ml. Predicted molecular weight ~39 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of FFPE human A431 cells with ABHD5 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam sections in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestinal cancer tissue with ABHD5 antibody at 1 ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
