H00051099-M01

antibody from Novus Biologicals

Targeting: ABHD5 CGI-58, NCIE2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00051099-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00051099-M01, RRID:AB_536536

Product name

Mouse Monoclonal Abhd5 Antibody

Antibody type

Monoclonal

Description

IgG purified. ABHD5 - abhydrolase domain containing 5

Reactivity

Human, Mouse, Rat, Bovine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Macrophage ABHD5 Suppresses NFκB-Dependent Matrix Metalloproteinase Expression and Cancer Metastasis.

Shang S, Ji X, Zhang L, Chen J, Li C, Shi R, Xiang W, Kang X, Zhang D, Yang F, Dai R, Chen P, Chen S, Chen Y, Li Y, Miao H
Cancer research 2019 Nov 1;79(21):5513-5526

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ABHD5 blunts the sensitivity of colorectal cancer to fluorouracil via promoting autophagic uracil yield.

Ou J, Peng Y, Yang W, Zhang Y, Hao J, Li F, Chen Y, Zhao Y, Xie X, Wu S, Zha L, Luo X, Xie G, Wang L, Sun W, Zhou Q, Li J, Liang H
Nature communications 2019 Mar 6;10(1):1078

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Loss of Abhd5 Promotes Colorectal Tumor Development and Progression by Inducing Aerobic Glycolysis and Epithelial-Mesenchymal Transition.

Ou J, Miao H, Ma Y, Guo F, Deng J, Wei X, Zhou J, Xie G, Shi H, Xue B, Liang H, Yu L
Cell reports 2018 Sep 4;24(10):2795-2797

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Stimulation of white adipose tissue lipolysis by xenin, a neurotensin-related peptide.

Bhavya S, Lew PS, Mizuno TM
Biochemical and biophysical research communications 2018 Apr 15;498(4):842-848

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Lipolysis in Brown Adipocytes Is Not Essential for Cold-Induced Thermogenesis in Mice.

Shin H, Ma Y, Chanturiya T, Cao Q, Wang Y, Kadegowda AKG, Jackson R, Rumore D, Xue B, Shi H, Gavrilova O, Yu L
Cell metabolism 2017 Nov 7;26(5):764-777.e5

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Central action of xenin affects the expression of lipid metabolism-related genes and proteins in mouse white adipose tissue.

Bhavya S, Lew PS, Mizuno TM
Neuropeptides 2017 Jun;63:67-73

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ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2.

Peng Y, Miao H, Wu S, Yang W, Zhang Y, Xie G, Xie X, Li J, Shi C, Ye L, Sun W, Wang L, Liang H, Ou J
Autophagy 2016 Nov;12(11):2167-2182

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Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.

Miao H, Ou J, Peng Y, Zhang X, Chen Y, Hao L, Xie G, Wang Z, Pang X, Ruan Z, Li J, Yu L, Xue B, Shi H, Shi C, Liang H
Nature communications 2016 May 18;7:11716

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ABHD5/CGI-58, the Chanarin-Dorfman Syndrome Protein, Mobilises Lipid Stores for Hepatitis C Virus Production.

Vieyres G, Welsch K, Gerold G, Gentzsch J, Kahl S, Vondran FW, Kaderali L, Pietschmann T
PLoS pathogens 2016 Apr;12(4):e1005568

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Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects.

Bakke SS, Feng YZ, Nikolić N, Kase ET, Moro C, Stensrud C, Damlien L, Ludahl MO, Sandbu R, Solheim BM, Rustan AC, Hjelmesæth J, Thoresen GH, Aas V
PloS one 2015;10(3):e0119556

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G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis.

Heier C, Radner FP, Moustafa T, Schreiber R, Grond S, Eichmann TO, Schweiger M, Schmidt A, Cerk IK, Oberer M, Theussl HC, Wojciechowski J, Penninger JM, Zimmermann R, Zechner R
The Journal of biological chemistry 2015 Oct 23;290(43):26141-50

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Muscle-specific deletion of comparative gene identification-58 (CGI-58) causes muscle steatosis but improves insulin sensitivity in male mice.

Xie P, Kadegowda AK, Ma Y, Guo F, Han X, Wang M, Groban L, Xue B, Shi H, Li H, Yu L
Endocrinology 2015 May;156(5):1648-58

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Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling.

Hofer P, Boeszoermenyi A, Jaeger D, Feiler U, Arthanari H, Mayer N, Zehender F, Rechberger G, Oberer M, Zimmermann R, Lass A, Haemmerle G, Breinbauer R, Zechner R, Preiss-Landl K
The Journal of biological chemistry 2015 Jul 24;290(30):18438-53

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Micro RNA-124a regulates lipolysis via adipose triglyceride lipase and comparative gene identification 58.

Das SK, Stadelmeyer E, Schauer S, Schwarz A, Strohmaier H, Claudel T, Zechner R, Hoefler G, Vesely PW
International journal of molecular sciences 2015 Apr 16;16(4):8555-68

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MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes.

Kurtz CL, Peck BC, Fannin EE, Beysen C, Miao J, Landstreet SR, Ding S, Turaga V, Lund PK, Turner S, Biddinger SB, Vickers KC, Sethupathy P
Diabetes 2014 Sep;63(9):3141-8

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Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.

Patel S, Yang W, Kozusko K, Saudek V, Savage DB
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 24;111(25):9163-8

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The hepatitis C virus core protein inhibits adipose triglyceride lipase (ATGL)-mediated lipid mobilization and enhances the ATGL interaction with comparative gene identification 58 (CGI-58) and lipid droplets.

Camus G, Schweiger M, Herker E, Harris C, Kondratowicz AS, Tsou CL, Farese RV Jr, Herath K, Previs SF, Roddy TP, Pinto S, Zechner R, Ott M
The Journal of biological chemistry 2014 Dec 26;289(52):35770-80

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Endurance exercise training up-regulates lipolytic proteins and reduces triglyceride content in skeletal muscle of obese subjects.

Louche K, Badin PM, Montastier E, Laurens C, Bourlier V, de Glisezinski I, Thalamas C, Viguerie N, Langin D, Moro C
The Journal of clinical endocrinology and metabolism 2013 Dec;98(12):4863-71

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Deficiency of liver Comparative Gene Identification-58 causes steatohepatitis and fibrosis in mice.

Guo F, Ma Y, Kadegowda AKG, Betters JL, Xie P, Liu G, Liu X, Miao H, Ou J, Su X, Zheng Z, Xue B, Shi H, Yu L
Journal of lipid research 2013 Aug;54(8):2109-2120

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Downregulation of adipose triglyceride lipase in the heart aggravates diabetic cardiomyopathy in db/db mice.

Inoue T, Kobayashi K, Inoguchi T, Sonoda N, Maeda Y, Hirata E, Fujimura Y, Miura D, Hirano K, Takayanagi R
Biochemical and biophysical research communications 2013 Aug 16;438(1):224-9

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Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier.

Pollak NM, Schweiger M, Jaeger D, Kolb D, Kumari M, Schreiber R, Kolleritsch S, Markolin P, Grabner GF, Heier C, Zierler KA, Rülicke T, Zimmermann R, Lass A, Zechner R, Haemmerle G
Journal of lipid research 2013 Apr;54(4):1092-102

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PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans.

Grall A, Guaguère E, Planchais S, Grond S, Bourrat E, Hausser I, Hitte C, Le Gallo M, Derbois C, Kim GJ, Lagoutte L, Degorce-Rubiales F, Radner FP, Thomas A, Küry S, Bensignor E, Fontaine J, Pin D, Zimmermann R, Zechner R, Lathrop M, Galibert F, André C, Fischer J
Nature genetics 2012 Jan 15;44(2):140-7

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Adipose-selective overexpression of ABHD5/CGI-58 does not increase lipolysis or protect against diet-induced obesity.

Caviglia JM, Betters JL, Dapito DH, Lord CC, Sullivan S, Chua S, Yin T, Sekowski A, Mu H, Shapiro L, Brown JM, Brasaemle DL
Journal of lipid research 2011 Nov;52(11):2032-42

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Liver X receptor (LXR) regulates human adipocyte lipolysis.

Stenson BM, Rydén M, Venteclef N, Dahlman I, Pettersson AM, Mairal A, Aström G, Blomqvist L, Wang V, Jocken JW, Clément K, Langin D, Arner P, Laurencikiene J
The Journal of biological chemistry 2011 Jan 7;286(1):370-9

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B56alpha/protein phosphatase 2A inhibits adipose lipolysis in high-fat diet-induced obese mice.

Kinney BP, Qiao L, Levaugh JM, Shao J
Endocrinology 2010 Aug;151(8):3624-32

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in HeLa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody (M01), clone 1F3.Lane 1: ABHD5 transfected lysate(39 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 expression in HeLa, mouse and bovine tissues. Primary: 1:500 dilution. This image was submitted via customer Review.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Abhd5 Antibody (1F3) [H00051099-M01] - Detection limit for recombinant GST tagged ABHD5 is approximately 1ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.