Antibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00051099-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051099-M01, RRID:AB_536536
- Product name
- Mouse Monoclonal Abhd5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ABHD5 - abhydrolase domain containing 5
- Reactivity
- Human, Mouse, Rat, Bovine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Macrophage ABHD5 Suppresses NFκB-Dependent Matrix Metalloproteinase Expression and Cancer Metastasis.
ABHD5 blunts the sensitivity of colorectal cancer to fluorouracil via promoting autophagic uracil yield.
Loss of Abhd5 Promotes Colorectal Tumor Development and Progression by Inducing Aerobic Glycolysis and Epithelial-Mesenchymal Transition.
Stimulation of white adipose tissue lipolysis by xenin, a neurotensin-related peptide.
Lipolysis in Brown Adipocytes Is Not Essential for Cold-Induced Thermogenesis in Mice.
Central action of xenin affects the expression of lipid metabolism-related genes and proteins in mouse white adipose tissue.
ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2.
Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.
ABHD5/CGI-58, the Chanarin-Dorfman Syndrome Protein, Mobilises Lipid Stores for Hepatitis C Virus Production.
Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects.
G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis.
Muscle-specific deletion of comparative gene identification-58 (CGI-58) causes muscle steatosis but improves insulin sensitivity in male mice.
Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling.
Micro RNA-124a regulates lipolysis via adipose triglyceride lipase and comparative gene identification 58.
MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes.
Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.
The hepatitis C virus core protein inhibits adipose triglyceride lipase (ATGL)-mediated lipid mobilization and enhances the ATGL interaction with comparative gene identification 58 (CGI-58) and lipid droplets.
Endurance exercise training up-regulates lipolytic proteins and reduces triglyceride content in skeletal muscle of obese subjects.
Deficiency of liver Comparative Gene Identification-58 causes steatohepatitis and fibrosis in mice.
Downregulation of adipose triglyceride lipase in the heart aggravates diabetic cardiomyopathy in db/db mice.
Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier.
PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans.
Adipose-selective overexpression of ABHD5/CGI-58 does not increase lipolysis or protect against diet-induced obesity.
Liver X receptor (LXR) regulates human adipocyte lipolysis.
B56alpha/protein phosphatase 2A inhibits adipose lipolysis in high-fat diet-induced obese mice.
Shang S, Ji X, Zhang L, Chen J, Li C, Shi R, Xiang W, Kang X, Zhang D, Yang F, Dai R, Chen P, Chen S, Chen Y, Li Y, Miao H
Cancer research 2019 Nov 1;79(21):5513-5526
Cancer research 2019 Nov 1;79(21):5513-5526
ABHD5 blunts the sensitivity of colorectal cancer to fluorouracil via promoting autophagic uracil yield.
Ou J, Peng Y, Yang W, Zhang Y, Hao J, Li F, Chen Y, Zhao Y, Xie X, Wu S, Zha L, Luo X, Xie G, Wang L, Sun W, Zhou Q, Li J, Liang H
Nature communications 2019 Mar 6;10(1):1078
Nature communications 2019 Mar 6;10(1):1078
Loss of Abhd5 Promotes Colorectal Tumor Development and Progression by Inducing Aerobic Glycolysis and Epithelial-Mesenchymal Transition.
Ou J, Miao H, Ma Y, Guo F, Deng J, Wei X, Zhou J, Xie G, Shi H, Xue B, Liang H, Yu L
Cell reports 2018 Sep 4;24(10):2795-2797
Cell reports 2018 Sep 4;24(10):2795-2797
Stimulation of white adipose tissue lipolysis by xenin, a neurotensin-related peptide.
Bhavya S, Lew PS, Mizuno TM
Biochemical and biophysical research communications 2018 Apr 15;498(4):842-848
Biochemical and biophysical research communications 2018 Apr 15;498(4):842-848
Lipolysis in Brown Adipocytes Is Not Essential for Cold-Induced Thermogenesis in Mice.
Shin H, Ma Y, Chanturiya T, Cao Q, Wang Y, Kadegowda AKG, Jackson R, Rumore D, Xue B, Shi H, Gavrilova O, Yu L
Cell metabolism 2017 Nov 7;26(5):764-777.e5
Cell metabolism 2017 Nov 7;26(5):764-777.e5
Central action of xenin affects the expression of lipid metabolism-related genes and proteins in mouse white adipose tissue.
Bhavya S, Lew PS, Mizuno TM
Neuropeptides 2017 Jun;63:67-73
Neuropeptides 2017 Jun;63:67-73
ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2.
Peng Y, Miao H, Wu S, Yang W, Zhang Y, Xie G, Xie X, Li J, Shi C, Ye L, Sun W, Wang L, Liang H, Ou J
Autophagy 2016 Nov;12(11):2167-2182
Autophagy 2016 Nov;12(11):2167-2182
Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM.
Miao H, Ou J, Peng Y, Zhang X, Chen Y, Hao L, Xie G, Wang Z, Pang X, Ruan Z, Li J, Yu L, Xue B, Shi H, Shi C, Liang H
Nature communications 2016 May 18;7:11716
Nature communications 2016 May 18;7:11716
ABHD5/CGI-58, the Chanarin-Dorfman Syndrome Protein, Mobilises Lipid Stores for Hepatitis C Virus Production.
Vieyres G, Welsch K, Gerold G, Gentzsch J, Kahl S, Vondran FW, Kaderali L, Pietschmann T
PLoS pathogens 2016 Apr;12(4):e1005568
PLoS pathogens 2016 Apr;12(4):e1005568
Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects.
Bakke SS, Feng YZ, Nikolić N, Kase ET, Moro C, Stensrud C, Damlien L, Ludahl MO, Sandbu R, Solheim BM, Rustan AC, Hjelmesæth J, Thoresen GH, Aas V
PloS one 2015;10(3):e0119556
PloS one 2015;10(3):e0119556
G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis.
Heier C, Radner FP, Moustafa T, Schreiber R, Grond S, Eichmann TO, Schweiger M, Schmidt A, Cerk IK, Oberer M, Theussl HC, Wojciechowski J, Penninger JM, Zimmermann R, Zechner R
The Journal of biological chemistry 2015 Oct 23;290(43):26141-50
The Journal of biological chemistry 2015 Oct 23;290(43):26141-50
Muscle-specific deletion of comparative gene identification-58 (CGI-58) causes muscle steatosis but improves insulin sensitivity in male mice.
Xie P, Kadegowda AK, Ma Y, Guo F, Han X, Wang M, Groban L, Xue B, Shi H, Li H, Yu L
Endocrinology 2015 May;156(5):1648-58
Endocrinology 2015 May;156(5):1648-58
Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling.
Hofer P, Boeszoermenyi A, Jaeger D, Feiler U, Arthanari H, Mayer N, Zehender F, Rechberger G, Oberer M, Zimmermann R, Lass A, Haemmerle G, Breinbauer R, Zechner R, Preiss-Landl K
The Journal of biological chemistry 2015 Jul 24;290(30):18438-53
The Journal of biological chemistry 2015 Jul 24;290(30):18438-53
Micro RNA-124a regulates lipolysis via adipose triglyceride lipase and comparative gene identification 58.
Das SK, Stadelmeyer E, Schauer S, Schwarz A, Strohmaier H, Claudel T, Zechner R, Hoefler G, Vesely PW
International journal of molecular sciences 2015 Apr 16;16(4):8555-68
International journal of molecular sciences 2015 Apr 16;16(4):8555-68
MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes.
Kurtz CL, Peck BC, Fannin EE, Beysen C, Miao J, Landstreet SR, Ding S, Turaga V, Lund PK, Turner S, Biddinger SB, Vickers KC, Sethupathy P
Diabetes 2014 Sep;63(9):3141-8
Diabetes 2014 Sep;63(9):3141-8
Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.
Patel S, Yang W, Kozusko K, Saudek V, Savage DB
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 24;111(25):9163-8
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 24;111(25):9163-8
The hepatitis C virus core protein inhibits adipose triglyceride lipase (ATGL)-mediated lipid mobilization and enhances the ATGL interaction with comparative gene identification 58 (CGI-58) and lipid droplets.
Camus G, Schweiger M, Herker E, Harris C, Kondratowicz AS, Tsou CL, Farese RV Jr, Herath K, Previs SF, Roddy TP, Pinto S, Zechner R, Ott M
The Journal of biological chemistry 2014 Dec 26;289(52):35770-80
The Journal of biological chemistry 2014 Dec 26;289(52):35770-80
Endurance exercise training up-regulates lipolytic proteins and reduces triglyceride content in skeletal muscle of obese subjects.
Louche K, Badin PM, Montastier E, Laurens C, Bourlier V, de Glisezinski I, Thalamas C, Viguerie N, Langin D, Moro C
The Journal of clinical endocrinology and metabolism 2013 Dec;98(12):4863-71
The Journal of clinical endocrinology and metabolism 2013 Dec;98(12):4863-71
Deficiency of liver Comparative Gene Identification-58 causes steatohepatitis and fibrosis in mice.
Guo F, Ma Y, Kadegowda AKG, Betters JL, Xie P, Liu G, Liu X, Miao H, Ou J, Su X, Zheng Z, Xue B, Shi H, Yu L
Journal of lipid research 2013 Aug;54(8):2109-2120
Journal of lipid research 2013 Aug;54(8):2109-2120
Downregulation of adipose triglyceride lipase in the heart aggravates diabetic cardiomyopathy in db/db mice.
Inoue T, Kobayashi K, Inoguchi T, Sonoda N, Maeda Y, Hirata E, Fujimura Y, Miura D, Hirano K, Takayanagi R
Biochemical and biophysical research communications 2013 Aug 16;438(1):224-9
Biochemical and biophysical research communications 2013 Aug 16;438(1):224-9
Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier.
Pollak NM, Schweiger M, Jaeger D, Kolb D, Kumari M, Schreiber R, Kolleritsch S, Markolin P, Grabner GF, Heier C, Zierler KA, Rülicke T, Zimmermann R, Lass A, Zechner R, Haemmerle G
Journal of lipid research 2013 Apr;54(4):1092-102
Journal of lipid research 2013 Apr;54(4):1092-102
PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans.
Grall A, Guaguère E, Planchais S, Grond S, Bourrat E, Hausser I, Hitte C, Le Gallo M, Derbois C, Kim GJ, Lagoutte L, Degorce-Rubiales F, Radner FP, Thomas A, Küry S, Bensignor E, Fontaine J, Pin D, Zimmermann R, Zechner R, Lathrop M, Galibert F, André C, Fischer J
Nature genetics 2012 Jan 15;44(2):140-7
Nature genetics 2012 Jan 15;44(2):140-7
Adipose-selective overexpression of ABHD5/CGI-58 does not increase lipolysis or protect against diet-induced obesity.
Caviglia JM, Betters JL, Dapito DH, Lord CC, Sullivan S, Chua S, Yin T, Sekowski A, Mu H, Shapiro L, Brown JM, Brasaemle DL
Journal of lipid research 2011 Nov;52(11):2032-42
Journal of lipid research 2011 Nov;52(11):2032-42
Liver X receptor (LXR) regulates human adipocyte lipolysis.
Stenson BM, Rydén M, Venteclef N, Dahlman I, Pettersson AM, Mairal A, Aström G, Blomqvist L, Wang V, Jocken JW, Clément K, Langin D, Arner P, Laurencikiene J
The Journal of biological chemistry 2011 Jan 7;286(1):370-9
The Journal of biological chemistry 2011 Jan 7;286(1):370-9
B56alpha/protein phosphatase 2A inhibits adipose lipolysis in high-fat diet-induced obese mice.
Kinney BP, Qiao L, Levaugh JM, Shao J
Endocrinology 2010 Aug;151(8):3624-32
Endocrinology 2010 Aug;151(8):3624-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in HeLa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody (M01), clone 1F3.Lane 1: ABHD5 transfected lysate(39 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 expression in HeLa, mouse and bovine tissues. Primary: 1:500 dilution. This image was submitted via customer Review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Abhd5 Antibody (1F3) [H00051099-M01] - Detection limit for recombinant GST tagged ABHD5 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.