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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-37669 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG4A Monoclonal Antibody (1458CT808.66.25.69)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1458CT808.66.25.69
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATG4A using the ATG4A Monoclonal Antibody (Product # MA5-37669) in K562 cells (lysate at 20 µg) at 1:500. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescent analysis of ATG4A in 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells using ATG4A Monoclonal Antibody (Product # MA5-37669) at a 1:25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red).The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ATG4A in human breast sections using the ATG4A Monoclonal Antibody (Product # MA5-37669) at a dilution of 1:25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis showing HeLa cells stained with ATG4A Monoclonal Antibody (Product # MA5-37669) at a 1:25 dilution (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by the ATG4A Monoclonal Antibody for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2b (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
