Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-38450 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG4A Monoclonal Antibody (8C9C2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8C9C2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using ATG4A Monoclonal Antibody (Product # MA5-38450) against human ATG4A (AA: 258-398) recombinant protein. (Expected MW is 42.2 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATG4A using ATG4A Monoclonal Antibody (Product # MA5-38450) in HEK293 (1) and ATG4A (AA: 258-398)-human IgG-Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of ATG4A in HeLa cells using ATG4A Monoclonal Antibody (Product # MA5-38450) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of ATG4A in HL-60 cells using ATG4A Monoclonal Antibody (Product # MA5-38450) (green) and negative control (red).