Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00084313-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00084313-M01, RRID:AB_426095
- Product name
- MGC10540 monoclonal antibody (M01), clone 2E5-2B9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant MGC10540.
- Antigen sequence
MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCS
LVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLP
VESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPE
EWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEE
FHGLDEATLLRALQALQQEHKAEIITVSDGRGVKF
F- Isotype
- IgG
- Antibody clone number
- 2E5-2B9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Western Blot analysis of MGC10540 expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged VPS25 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to VPS25 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
