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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6937 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6937, RRID:AB_1581868
- Product name
- VPS25 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of VPS25.
- Antigen sequence
C-QPNVDTRQKQ- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The Drosophila tumor suppressor vps25 prevents nonautonomous overproliferation by regulating notch trafficking.
Vaccari T, Bilder D
Developmental cell 2005 Nov;9(5):687-98
Developmental cell 2005 Nov;9(5):687-98
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VPS25 polyclonal antibody (Cat # PAB6937) (0.5 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VPS25 polyclonal antibody (Cat # PAB6937) (10 ug/mL) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
