Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-2468 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2468, RRID:AB_2215020
- Product name
- Goat Polyclonal VPS25 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The Drosophila tumor suppressor vps25 prevents nonautonomous overproliferation by regulating notch trafficking.
Vaccari T, Bilder D
Developmental cell 2005 Nov;9(5):687-98
Developmental cell 2005 Nov;9(5):687-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VPS25 Antibody [NB100-2468] - (0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468] - (10ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.