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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NB600-284 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-284, RRID:AB_10001015
- Product name
- Rabbit Polyclonal WNK4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Sympathetic regulation of NCC in norepinephrine-evoked salt-sensitive hypertension in Sprague-Dawley rats.
Role of nitric oxide and WNK-SPAK/OSR1-KCC2 signaling in daily changes in GABAergic inhibition in the rat dorsal raphe neurons.
High frequency and wide range of human kidney papillary crystalline plugs.
WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats.
Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl- cotransporter activity and expression in male and female mice.
FGF23 promotes renal calcium reabsorption through the TRPV5 channel.
Immunolocalization of WNK4 in mouse kidney.
Pathophysiological roles of WNK kinases in the kidney.
WNK4 diverts the thiazide-sensitive NaCl cotransporter to the lysosome and stimulates AP-3 interaction.
Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats.
WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia.
Frame AA, Puleo F, Kim K, Walsh KR, Faudoa E, Hoover RS, Wainford RD
American journal of physiology. Renal physiology 2019 Dec 1;317(6):F1623-F1636
American journal of physiology. Renal physiology 2019 Dec 1;317(6):F1623-F1636
Role of nitric oxide and WNK-SPAK/OSR1-KCC2 signaling in daily changes in GABAergic inhibition in the rat dorsal raphe neurons.
Kim MJ, Yang HJ, Kim Y, Kang I, Kim SS, Cho YW
Neuropharmacology 2018 Jun;135:355-367
Neuropharmacology 2018 Jun;135:355-367
High frequency and wide range of human kidney papillary crystalline plugs.
Huguet L, Le Dudal M, Livrozet M, Bazin D, Frochot V, Perez J, Haymann JP, Brocheriou I, Daudon M, Letavernier E
Urolithiasis 2018 Aug;46(4):333-341
Urolithiasis 2018 Aug;46(4):333-341
WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic brain damage in hypertensive rats.
Bhuiyan MIH, Song S, Yuan H, Begum G, Kofler J, Kahle KT, Yang SS, Lin SH, Alper SL, Subramanya AR, Sun D
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2017 Aug;37(8):2780-2794
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2017 Aug;37(8):2780-2794
Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl- cotransporter activity and expression in male and female mice.
Li J, Hatano R, Xu S, Wan L, Yang L, Weinstein AM, Palmer L, Wang T
American journal of physiology. Renal physiology 2017 Aug 1;313(2):F505-F513
American journal of physiology. Renal physiology 2017 Aug 1;313(2):F505-F513
FGF23 promotes renal calcium reabsorption through the TRPV5 channel.
Andrukhova O, Smorodchenko A, Egerbacher M, Streicher C, Zeitz U, Goetz R, Shalhoub V, Mohammadi M, Pohl EE, Lanske B, Erben RG
The EMBO journal 2014 Feb 3;33(3):229-46
The EMBO journal 2014 Feb 3;33(3):229-46
Immunolocalization of WNK4 in mouse kidney.
Ohno M, Uchida K, Ohashi T, Nitta K, Ohta A, Chiga M, Sasaki S, Uchida S
Histochemistry and cell biology 2011 Jul;136(1):25-35
Histochemistry and cell biology 2011 Jul;136(1):25-35
Pathophysiological roles of WNK kinases in the kidney.
Uchida S
Pflugers Archiv : European journal of physiology 2010 Sep;460(4):695-702
Pflugers Archiv : European journal of physiology 2010 Sep;460(4):695-702
WNK4 diverts the thiazide-sensitive NaCl cotransporter to the lysosome and stimulates AP-3 interaction.
Subramanya AR, Liu J, Ellison DH, Wade JB, Welling PA
The Journal of biological chemistry 2009 Jul 3;284(27):18471-80
The Journal of biological chemistry 2009 Jul 3;284(27):18471-80
Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats.
Song J, Hu X, Riazi S, Tiwari S, Wade JB, Ecelbarger CA
American journal of physiology. Renal physiology 2006 May;290(5):F1055-64
American journal of physiology. Renal physiology 2006 May;290(5):F1055-64
WNK4 regulates apical and basolateral Cl- flux in extrarenal epithelia.
Kahle KT, Gimenez I, Hassan H, Wilson FH, Wong RD, Forbush B, Aronson PS, Lifton RP
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2064-9
Proceedings of the National Academy of Sciences of the United States of America 2004 Feb 17;101(7):2064-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WNK4 Antibody [NB600-284] - Western Blot Image of anti-WNK4. Whole cell protein from Hek293 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WNK4 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence
