Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-30068 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal EDNRA/Endothelin R Type A Antibody
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 2 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EDNRA/Endothelin R Type A Antibody [NBP2-30068] - Western blot analysis of EDNRA antibody (Center) (NBP2-30068) in CEM cell line lysates (35ug/lane). EDNRA (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: EDNRA/Endothelin R Type A Antibody [NBP2-30068] - Confocal immunofluorescent analysis of EDNRA Antibody (Center)(NBP2-30068) with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EDNRA/Endothelin R Type A Antibody [NBP2-30068] - Flow cytometric analysis of CEM cells using EDNRA Antibody (Center) (green histogram) compared to a negative control cell (blue histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.