- PA3-065 - Invitrogen Antibodies EDNRA antibody

Submitted references Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line.

Torres MJ, López-Moncada F, Herrera D, Indo S, Lefian A, Llanos P, Tapia J, Castellón EA, Contreras HR
Oncology reports 2021 Aug;46(2)

Peroxisome proliferator-activated receptor gamma blunts endothelin-1-mediated contraction of the uterine artery in a murine model of high-altitude pregnancy.

Lane SL, Doyle AS, Bales ES, Houck JA, Lorca RA, Moore LG, Julian CG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Mar;34(3):4283-4292

Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8.

Lupp A, Mann A, Heeb A, Kaemmerer D, Sänger J, Evert M, Evert K, Mawrin C, Schulz S
Peptides 2015 Apr;66:19-25

Transforming growth factor-β regulates endothelin-1 signaling in the newborn mouse lung during hypoxia exposure.

Olave N, Nicola T, Zhang W, Bulger A, James M, Oparil S, Chen YF, Ambalavanan N
American journal of physiology. Lung cellular and molecular physiology 2012 May 1;302(9):L857-65

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Endothelin A Receptor in mouse heart using a polyclonal antibody (Product # PA3-065).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Endothelin A Receptor Polyclonal Antibody (Product # PA3-065) and a 50kDa band corresponding to Endothelin A Receptor was observed across tissues tested. Tissue extracts (40 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Lung (Lane 5), Rat Lung (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Endothelin A Receptor in ETA-transfected cells using a polyclonal antibody (Product # PA3-065).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Endothelin A Receptor in ETA-transfected cells using a polyclonal antibody (Product # PA3-065).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of ETA in mouse dorsal root ganglia

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of ETA receptors in the atrium of mouse heart.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on acetone-fixed frozen mouse skin tissue sections. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (panel A), and Endothelin Receptor A polyclonal antibody (Product # PA3-065, panel B), or an Endothelin Receptor B polyclonal antibody (Product # PA3-066, panel C) all at dilutions of 1:800 overnight at 4C. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy. Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on acetone-fixed frozen mouse skin tissue sections. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (panel A), and Endothelin Receptor A polyclonal antibody (Product # PA3-065, panel B), or an Endothelin Receptor B polyclonal antibody (Product # PA3-066, panel C) all at dilutions of 1:800 overnight at 4C. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy. Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. ET A R and ET B R expression in prostate cancer cells. (A) ET A R mRNA expression normalized to Pumilio compared with PC3 cells. (B) ET A R protein expression in PC3 and LNCaP cells. Quantification was normalized to beta-actin and PC3 cells. (C) ET B R mRNA expression normalized to Pumilio compared with LNCaP cells. (D) ET B R protein expression in PC3 and LNCaP cells normalized to Pumilio compared with LNCaP cells. Immunofluorescence analysis of (E) ET A R and (F) ET B R in PC3 and LNCaP cells. (G) Negative controls. Data are presented as the mean +- SD (n=3 independent experiments) of arbitrary units. *P

PA3-065

Antibody data