CABT-B9206

antibody from Creative Diagnostics

Targeting: EDNRA ET-A, ETA-R, hET-AR

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9206

Provider

Creative Diagnostics

Product name

Mouse anti-Rat Endothelin 1 Receptor monoclonal antibody, clone 27

Antibody type

Monoclonal

Description

Endothelin-1 (ET-1) is a potent vasoconstrictor that is secreted by vascular endothelial cells. ET-1, ET-2, ET-3, and endothelin ÉÇ are members of a family of related peptides. All are 21 amino acid peptides with two disulfide bridges. ET-1 and ET-2 differ by only two residues, while ET-3 differs from both by six residues. ET-1 and ET-2 are highly effective on vascular smooth muscle, while the effects of ET-3 are minimal. ET-1 and its precursor, big ET-1, are the primary forms present in the circulation. Circulating ET-1 plays a pathologic role in the etiology of hypertension and antagonists are utilized in the treatment of this disease. The ET-1 receptor is a seven-transmembrane-domain receptor of the G-protein-coupled family that signals via transient increases in intracellular Ca2+. It is primarily expressed in lung and heart and is detected at lower levels in liver, brain, muscle, and kidney. ET-1 receptor interacts with ET-1, ET-2, and ET-3. However, it has a 2-3 fold higher affinity for ET-1 than ET-2 and the affinity for ET-3 is more than 100 fold lower. Thus, ligation of the ET-1 receptor by ET-1 induces increased intracellular Ca2+ that signals to promote vasoconstriction.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

27

Vial size

50 _g

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.