PA5-104508

antibody from Invitrogen Antibodies

Targeting: COL4A6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-104508

Provider

Invitrogen Antibodies

Product name

Collagen IV Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total Collagen IV.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Collagen IV in 293 and HeLa (Lane 1: 293 cells, Lane 2: Hela cells, Lane 3: 293 cells treated with blocking peptide). Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-104508).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Collagen IV in 293 and HeLa (Lane 1: 293 cells, Lane 2: Hela cells, Lane 3: 293 cells treated with blocking peptide). Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-104508).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Collagen IV in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Collagen IV polyclonal antibody (Product # PA5-104508) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Collagen IV in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Collagen IV polyclonal antibody (Product # PA5-104508) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Collagen IV in human colon cancer tissues sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Collagen IV polyclonal antibody (Product # PA5-104508) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Collagen IV in human Melanoma tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Collagen IV polyclonal antibody (Product # PA5-104508) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 2 TIMP3 overexpression in macrophages reduces STZ-induced inflammation and fibrosis in kidney. Kidney cortex analysis of (A) gene expression of F4/80, MCP1, TNF-alpha, IL6, and COL1A2 and (B) protein levels of WT1, Podocin, Nephrin, Coll-IV, TGF-beta, Actin, phospho-p38 (p-p38), p38 in wt, and MacT3 non-diabetic and diabetic mice (n = 6 per group). (C) Protein levels of WT1, Podocin, and Actin in kidney glomerular fraction of wt and MacT3 non-diabetic and diabetic mice (n = 5 per group). A representative image of two mice per group is shown. Relative protein levels as determined by densitometry (*p < 0.05, **p

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 4 Podocyte deletion of ADAM17 attenuates STZ-induced injury in kidney. Twelve weeks after STZ administration diabetic Ct and DeltaPodA17 mice and age-matched non-diabetic littermates (Ctrl) were analyzed for (A) PAS-staining in kidney and quantification of mGA, fMA, and mMA (arrows indicate glomerular hypertrophy and mesangial expansion) and (B) immunohistochemical staining of Nox4 and Coll-IV in kidney (non-diabetic n = 5, diabetic n = 10 per group, original magnification, X400) (arrows indicate increased glomerular staining for Nox4 and Coll-IV). (C) Protein expression of WT1, Podocin, Nephrin, Coll-IV, and Actin in kidney cortex from Ct and DeltaPodA17 non-diabetic and diabetic mice (n = 6 per group). A representative image of two mice per group is shown. Relative protein levels as determined by densitometry (*p < 0.05, **p