AGC-006-25UL

antibody from Invitrogen Antibodies

Targeting: GRM1 GPRC1A, mGlu1, MGLUR1, PPP1R85

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

AGC-006-25UL

Provider

Invitrogen Antibodies

Product name

mGluR1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain lysate: - 1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200). 2. Anti-mGluR1 (extracellular) Antibody , preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof mouse brain lysate: - 1. Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:200). 2. Anti-mGluR1 (extracellular) Antibody , preincubated with mGluR1 (extracellular) Blocking Peptide (#BLP-GC006).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of mGluR1 in rat C6 glioma cells - Cell surface detection of mGluR1 in live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:100), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red). Nuclei were stained with Hoechst 33342 (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of mGluR1 in rat C6 glioma cells - Cell surface detection of mGluR1 in live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) Antibody (#AGC-006), (1:100), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red). Nuclei were stained with Hoechst 33342 (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of mGluR1 in live intact Jurkat cells: - (black) Unstained cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-mGluR1 (extracellular) Antibody (#AGC-006), (10 µg) + goat- Anti-rabbit-FITC.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of rat brain lysate: - 1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) Antibody (#AGC-006). 2. Cell lysate + protein A beads + pre-immune rabbit serum.3. Cell lysate. Black arrow indicates the mGluR1 protein (glycosylated form) while the red arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-mGluR1 (extracellular) Antibody .