Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- MAB3654 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GPR15 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains human GPR15-transfected cells but not irrelevant transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P49685
- Isotype
- IgG
- Antibody clone number
- 367902
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tobacco-smoking induced GPR15-expressing T cells in blood do not indicate pulmonary damage.
Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge.
CXCR6-Mediated Simian Immunodeficiency Virus SIVagmSab Entry into Sabaeus African Green Monkey Lymphocytes Implicates Widespread Use of Non-CCR5 Pathways in Natural Host Infections.
Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo.
Role and species-specific expression of colon T cell homing receptor GPR15 in colitis.
Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis.
Role of C-terminal membrane-proximal basic residues in cell surface trafficking of HIV coreceptor GPR15 protein.
The role of the alternative coreceptor GPR15 in SIV tropism for human cells.
Phosphorylation-dependent C-terminal binding of 14-3-3 proteins promotes cell surface expression of HIV co-receptor GPR15.
Bauer M, Fink B, Seyfarth HJ, Wirtz H, Frille A
BMC pulmonary medicine 2017 Nov 28;17(1):159
BMC pulmonary medicine 2017 Nov 28;17(1):159
Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge.
Seong Y, Lazarus NH, Sutherland L, Habtezion A, Abramson T, He XS, Greenberg HB, Butcher EC
JCI insight 2017 Mar 23;2(6):e90233
JCI insight 2017 Mar 23;2(6):e90233
CXCR6-Mediated Simian Immunodeficiency Virus SIVagmSab Entry into Sabaeus African Green Monkey Lymphocytes Implicates Widespread Use of Non-CCR5 Pathways in Natural Host Infections.
Wetzel KS, Yi Y, Elliott STC, Romero D, Jacquelin B, Hahn BH, Muller-Trutwin M, Apetrei C, Pandrea I, Collman RG
Journal of virology 2017 Feb 15;91(4)
Journal of virology 2017 Feb 15;91(4)
Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo.
Fischer A, Zundler S, Atreya R, Rath T, Voskens C, Hirschmann S, López-Posadas R, Watson A, Becker C, Schuler G, Neufert C, Atreya I, Neurath MF
Gut 2016 Oct;65(10):1642-64
Gut 2016 Oct;65(10):1642-64
Role and species-specific expression of colon T cell homing receptor GPR15 in colitis.
Nguyen LP, Pan J, Dinh TT, Hadeiba H, O'Hara E 3rd, Ebtikar A, Hertweck A, Gökmen MR, Lord GM, Jenner RG, Butcher EC, Habtezion A
Nature immunology 2015 Feb;16(2):207-213
Nature immunology 2015 Feb;16(2):207-213
Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis.
Cartwright A, Schmutz C, Askari A, Kuiper JH, Middleton J
Cytokine 2014 Jun;67(2):53-9
Cytokine 2014 Jun;67(2):53-9
Role of C-terminal membrane-proximal basic residues in cell surface trafficking of HIV coreceptor GPR15 protein.
Okamoto Y, Bernstein JD, Shikano S
The Journal of biological chemistry 2013 Mar 29;288(13):9189-99
The Journal of biological chemistry 2013 Mar 29;288(13):9189-99
The role of the alternative coreceptor GPR15 in SIV tropism for human cells.
Kiene M, Marzi A, Urbanczyk A, Bertram S, Fisch T, Nehlmeier I, Gnirss K, Karsten CB, Palesch D, Münch J, Chiodi F, Pöhlmann S, Steffen I
Virology 2012 Nov 10;433(1):73-84
Virology 2012 Nov 10;433(1):73-84
Phosphorylation-dependent C-terminal binding of 14-3-3 proteins promotes cell surface expression of HIV co-receptor GPR15.
Okamoto Y, Shikano S
The Journal of biological chemistry 2011 Mar 4;286(9):7171-81
The Journal of biological chemistry 2011 Mar 4;286(9):7171-81
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of GPR15 in HEK293 Human Cell Line Transfected with human GRP15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GRP15 and eGFP was stained with and either (A) Mouse Anti-Human GPR15 Monoclonal Antibody (Catalog # MAB3654) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).