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Learn60004-1-IG
antibody from Invitrogen Antibodies
Targeting: GAPDHS
GAPD2, GAPDH-2, GAPDS
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [8]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 60004-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAPDH Monoclonal Antibody (1E6D9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Yeast, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E6D9
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-IG at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Zebrafish tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Hela cell with anti-GAPDH (60004-1-IG) at various dilutions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-IG ( GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Soybean whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Whole yeast cells were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-IG (GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 60004-1-IG (GAPDH antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 60004-1-Ig (GAPDH Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 60004-1-Ig (GAPDH Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 60004-1-Ig (GAPDH antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 60004-1-Ig (GAPDH antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-IG ( GAPDH Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 60004-1-IG ( GAPDH Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug GAPDH antibody (60004-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IGG with dilution 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-GAPDH (60004-1-IG for IP and Detection) with HeLa cell lysate.
