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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB100-2421 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2421, RRID:AB_10001541
- Product name
- Goat Polyclonal DARC Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_001116423.1
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Chemokine (C-C motif) ligand 2 and coronary artery disease: Tissue expression of functional and atypical receptors.
Distinct Transcript Isoforms of the Atypical Chemokine Receptor 1 (ACKR1)/Duffy Antigen Receptor for Chemokines (DARC) Gene Are Expressed in Lymphoblasts and Altered Isoform Levels Are Associated with Genetic Ancestry and the Duffy-Null Allele.
Host genotype and tumor phenotype of chemokine decoy receptors integrally affect breast cancer relapse.
Absence of multiple atypical chemokine binders (ACBs) and the presence of VEGF and MMP-9 predict axillary lymph node metastasis in early breast carcinomas.
Coexpression of atypical chemokine binders (ACBs) in breast cancer predicts better outcomes.
Expression of DARC, CXCR3 and CCR5 in giant cell arteritis.
Hernández-Aguilera A, Fibla M, Cabré N, Luciano-Mateo F, Camps J, Fernández-Arroyo S, Martín-Paredero V, Menendez JA, Sirvent JJ, Joven J
Cytokine 2020 Feb;126:154923
Cytokine 2020 Feb;126:154923
Distinct Transcript Isoforms of the Atypical Chemokine Receptor 1 (ACKR1)/Duffy Antigen Receptor for Chemokines (DARC) Gene Are Expressed in Lymphoblasts and Altered Isoform Levels Are Associated with Genetic Ancestry and the Duffy-Null Allele.
Davis MB, Walens A, Hire R, Mumin K, Brown AM, Ford D, Howerth EW, Monteil M
PloS one 2015;10(10):e0140098
PloS one 2015;10(10):e0140098
Host genotype and tumor phenotype of chemokine decoy receptors integrally affect breast cancer relapse.
Yu KD, Wang X, Yang C, Zeng XH, Shao ZM
Oncotarget 2015 Sep 22;6(28):26519-27
Oncotarget 2015 Sep 22;6(28):26519-27
Absence of multiple atypical chemokine binders (ACBs) and the presence of VEGF and MMP-9 predict axillary lymph node metastasis in early breast carcinomas.
Zeng XH, Ou ZL, Yu KD, Feng LY, Yin WJ, Li J, Shen ZZ, Shao ZM
Medical oncology (Northwood, London, England) 2014 Sep;31(9):145
Medical oncology (Northwood, London, England) 2014 Sep;31(9):145
Coexpression of atypical chemokine binders (ACBs) in breast cancer predicts better outcomes.
Zeng XH, Ou ZL, Yu KD, Feng LY, Yin WJ, Li J, Shen ZZ, Shao ZM
Breast cancer research and treatment 2011 Feb;125(3):715-27
Breast cancer research and treatment 2011 Feb;125(3):715-27
Expression of DARC, CXCR3 and CCR5 in giant cell arteritis.
Brühl H, Vielhauer V, Weiss M, Mack M, Schlöndorff D, Segerer S
Rheumatology (Oxford, England) 2005 Mar;44(3):309-13
Rheumatology (Oxford, England) 2005 Mar;44(3):309-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
