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LearnSTJ70779
antibody from St John's Laboratory
Targeting: ACKR1
CCBP1, CD234, DARC, Dfy, FY, GPD
Western blot ELISA Immunocytochemistry Immunohistochemistry
Flow cytometry Immunoelectron microscopy Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- STJ70779 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-Duffy/FY/DARC antibody (N-Term) (STJ70779)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-Duffy/FY/DARC (N-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and EM research applications.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
HRAELSPSTENSSQLDFED- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0. 4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70779 (0. 3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70779 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
