AMR-024-200UL

antibody from Invitrogen Antibodies

Targeting: MC4R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMR-024-200UL

Provider

Invitrogen Antibodies

Product name

MC4 Receptor (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain lysates: - 1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400). 2. Anti-MC4 Receptor (extracellular) Antibody , preincubated with MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse brain lysates: - 1. Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:400). 2. Anti-MC4 Receptor (extracellular) Antibody , preincubated with MC4 Receptor (extracellular) Blocking Peptide (#BLP-MR024).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of MC4R inrat pituitary cell line - Cell surface detection of MC4R in live intact GH3 cells with Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:50). A. MC4R staining was observed,followed by Alexa-555-conjugated goat Anti-rabbit secondary Antibody (red).Hoechst 33342 (blue) is used to visualize the nuclei. B. Live view of the same field as A.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of MC4R inrat pituitary cell line - Cell surface detection of MC4R in live intact GH3 cells with Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:50). A. MC4R staining was observed,followed by Alexa-555-conjugated goat Anti-rabbit secondary Antibody (red).Hoechst 33342 (blue) is used to visualize the nuclei. B. Live view of the same field as A.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of MC4R in mouse brain - Immunohistochemical staining of perfusion-fixed frozen mouse brainsections using Anti-MC4 Receptor (extracellular) Antibody (#AMR-024), (1:100). MC4R (green)is expressedin the mouse hypothalamus in axonal processes (arrows). Hoechst 33342is used asthe counterstain (blue).