Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-68309 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTPRB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Nxhl Controls Angiogenesis by Targeting VE-PTP Through Interaction With Nucleolin.
Luo H, Zhang Y, Deng Y, Li L, Sheng Z, Yu Y, Lin Y, Chen X, Feng P
Frontiers in cell and developmental biology 2021;9:728821
Frontiers in cell and developmental biology 2021;9:728821
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTPRB in HuvEc whole cell lysates using a PTPRB Polyclonal Antibody (Product # PA5-68309).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 6 Nxhl regulates VE-PTP ( ptprb ) through interactions with NCL. (A) ChIRP-MS identification of nxhl RNA binding proteins using zebrafish tissues. qPCR identification of nxhl RNA in the eluted RNAs. Graph shows more than 90% nxhl RNA was retrieved, and no GAPDH was detected. Heat map shows major proteins are enriched and significantly (change fold > 2 and p < 0.05) retrieved by nxhl and control probes, analyzed by LC/MS-MS. NCL protein (purple boxed) was selected as candidate for follow-up study. The Centroid of NCL protein shows that NCL protein is pulled down and identified by LC/MS-MS. The specific peptide identifies NCL protein. (B) The interaction between nxhl , VE-PTP mRNA and NCL protein by RIP-qPCR assay. The mRNA expression of nxhl was determined by qPCR and Western blotting against Flag antibody and used to identify the successful expression of pcDNA3.1- Flag- nxhl plasmid in 293T cells. Bars show the interaction between nxhl mRNA and NCL protein. The interaction between VE-PTP mRNA and NCL protein is shown too, and qPCR shows the detection for VE-PTP mRNA expression in the NCL-pulled down RNA. (C) The interaction between nxhl , VE-PTP mRNA and NCL protein by pull down assay. Gels show the interaction between VE-PTP mRNA and NCL protein. Western blotting was performed to detect NCL protein in the VE-PTP-biotin probe -pulled down proteins in 293T cells. The interaction between ptprb mRNA and NCL protein is shown too. (D) Loss of nxhl affects the expression of