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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Other assay [3]
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- Product number
- PA5-31400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RUFY1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, U87-MG, MCF-7, mouse adipose.
- Concentration
- 1 mg/mL
Submitted references Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor-dependent manner.
Gosney JA, Wilkey DW, Merchant ML, Ceresa BP
The Journal of biological chemistry 2018 Apr 20;293(16):5895-5908
The Journal of biological chemistry 2018 Apr 20;293(16):5895-5908
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RUFY1 Polyclonal Antibody (Product # PA5-31400). Sample (50 µg of whole cell lysate). Lane A: Mouse brown adipose tissue . 7.5% SDS PAGE. RUFY1 Polyclonal Antibody (Product # PA5-31400) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RUFY1 Polyclonal Antibody (Product # PA5-31400). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 7.5% SDS PAGE. RUFY1 Polyclonal Antibody (Product # PA5-31400) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RUFY1 in methanol-fixed MCF-7 cells using a RUFY1 polyclonal antibody (Product # PA5-31400) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Immunoblot validation of candidate proteins from immunoisolated early endosomes. HeLa cells treated with +-10 ng/ml EGF were subjected to Percoll gradient fractionation and EEA1-targeted immunoisolation as described under ""Experimental procedures."" A , Percoll gradient fractions were resolved on a 10% SDS-PAGE and immunoblotted for EEA1, PTPN23, RUFY1, or STOML2. B , quantification of the immunoblots in A .
