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Learn11468-1-AP
antibody from Invitrogen Antibodies
Targeting: ADRM1
ARM1, GP110, Rpn13
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 11468-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADRM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTTSGALFP SLVPGSRGAS NKYLVEFRAG KMSLKGTTVT PDKRKGLVYI QQTDDSLIHF CWKDRTSGNV EDDLIIFPDD CEFKRVPQCP SGRVYVLKFK AGSKRLFFWM QEPKTDQDEE HCRKVNEYLN NPPMPGALGA SGSSGHELSA LGGEGGLQSL LGNMSHSQLM QLIGPAGLGG LGGLGALTGP GLASLLGSSG PPGSSSSSSS RSQSAAVTPS STTSSTRATP APSAPAAASA TSPSPAPSSG NGASTAASPT QPIQLSDLQS ILATMNVPAG PAGGQQVDLA SVLTPEIMAP ILANADVQER LLPYLPSGES LPQTADEIQN TLTSPQFQQA LGMFSAALAS GQLGPLMCQF GLPAEAVEAA NKGDVEAFAK AMQNNAKPEQ KEGDTKDKKD EEEDMSLD (1-407 aa encoded by BC017245)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using ADRM1 antibody 11468-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11468-1-AP (ADRM1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11468-1-AP (ADRM1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug ADRM1 antibody (11468-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ADRM1 (IP:11468-1-AP, 3ug; Detection:11468-1-AP 1:500) with mouse testis tissue lysate 8000ug.
