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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Other assay [3]
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- Product number
- PA5-57305 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIF15 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ESLLATEKVI SSLEKSRDSD KKVVADLMNQ IQELRTSVCE KTETIDTLKQ ELKDINCKYN SALVDREESR VLIKKQEVDI LD
- Concentration
- 0.1 mg/mL
Submitted references KIF15 Promotes Proliferation and Growth of Hepatocellular Carcinoma.
Sun YF, Wu HL, Shi RF, Chen L, Meng C
Analytical cellular pathology (Amsterdam) 2020;2020:6403012
Analytical cellular pathology (Amsterdam) 2020;2020:6403012
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KIF15 in human cell line MOLT-4 using a KIF15 Polyclonal Antibody (Product # PA5-57305).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KIF15 in human testis using KIF15 Polyclonal Antibody (Product # PA5-57305) shows moderate cytoplasmic positivity in Leydig cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KIF15 in human rectum using KIF15 Polyclonal Antibody (Product # PA5-57305) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KIF15 in human small intestine using KIF15 Polyclonal Antibody (Product # PA5-57305) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KIF15 in human skeletal muscle using KIF15 Polyclonal Antibody (Product # PA5-57305) shows no cytoplasmic positivity in myocytes as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 KIF15 expression was associated with poor prognosis of patients who underwent HCC. (a) Immunohistochemical staining of KIF15 protein in human HCC tissues (x100 and x200 magnification, respectively). (b) Immunohistochemical staining of KIF15 in the corresponding adjacent tissues (x100 and x200 magnification, respectively).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 KIF15 expression was effectively inhibited in both Hep3B and SNU-475 human HCC cells caused by its shRNA plasmids. (a) Quantitative PCR assays revealed the dramatically reduced expression level of KIF15 caused by its shRNA in Hep3B and SNU-475 cells, respectively. (b) Immunoblot assays confirmed the efficiently silencing of KIF15 caused by the transfection of its shRNA plasmids in Hep3B and SNU-475 cells. Results are presented as mean +- SD, * P < 0.05.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 KIF15 facilitated HCC growth in mice. (a) Hep3B cells infected with control or KIF15 shRNA lentivirus were subcutaneously implanted into nude mice. After 2 weeks, tumors were isolated, and volume was examined every 3 days ( n = 4 in each group). Tumor growth curve was calculated and analyzed according to the average volume of 4 tumors in each group. (b) IHC assays indicated the expression level of KIF15 in control or KIF15 depletion tumor tissues isolated from mice. (c) IHC assays revealed the expression level of Ki67 in control or KIF15 depletion tumor tissues taken from mice. Results are presented as mean +- SD, * P < 0.05.
