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- Product number
- PA5-43777 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: PPAPGPGQLT LRLRACGLNF ADLMARQGLY DRLPPLPVTP GMEGAGVVIA
- Concentration
- 0.5 mg/mL
Submitted references Chemical Proteomics Reveals Off-Targets of the Anandamide Reuptake Inhibitor WOBE437.
Gagestein B, Stevens AF, Fazio D, Florea BI, van der Wel T, Bakker AT, Fezza F, Dulk HD, Overkleeft HS, Maccarrone M, van der Stelt M
ACS chemical biology 2022 May 20;17(5):1174-1183
ACS chemical biology 2022 May 20;17(5):1174-1183
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cells using an anti-VAT1 polyclonal antibody (Product # PA5-43777).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cell lysate using an anti-VAT1 polyclonal antibody (Product # PA5-43777).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Partial SCCPDH and VAT1 knockouts were generated by CRISPR-Cas9. (A) SCCPDH and (C) VAT1 KO Neuro-2a lines were generated and checked by gel-based AfBPP using 0.1 muM pac-WOBE (3) for residual expression. VAT1 protein was tested by VAT1 Western blot. Coomassie served as a protein loading control. Lipid levels were tested by lipidomics on (B) SCCPDH KO and (D) VAT1 KO cells and compared to WT cells. Further characterization and the complete list of ratios are depicted in Figures S6 and S7 . (E) Neuro-2a cells were treated with 10 muM WOBE437 or vehicle for 30 min and harvested to be analyzed by MS-based lipidomics. Lipid levels are displayed as ratio against the same type of cells treated with vehicle. Data represent means +- SEM ( n = 4). One-way ANOVA with Dunnett's multiple comparisons correction: not significant when compared to WT.
