HPA038976

antibody from Atlas Antibodies

Targeting: DEF6 IBP, SLAT, SWAP70L

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA038976

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA038976, RRID:AB_10673409

Product name

Anti-DEF6

Antibody type

Polyclonal

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RADEDVEAAQRKLRQASTNVKHWNVQMNRLMHPIE
PGDKRPVTSSSFSGFQPPLLAHRDSSLKRLTRWGS
QGNRTPSPNSNEQQKSLNGG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines A-431 and A-549 using Anti-DEF6 antibody. Corresponding DEF6 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Sample type

RAT

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human lymph node and kidney tissues using Anti-DEF6 antibody. Corresponding DEF6 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows low expression as expected.

Sample type

HUMAN