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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00050619-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00050619-M01, RRID:AB_464088
- Product name
- DEF6 monoclonal antibody (M01), clone 1F2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant DEF6.
- Antigen sequence
MALRKELLKSIWYAFTALDVEKSGKVSKSQLKVLS
HNLYTVLHIPHDPVALEEHFRDDDDGPVSSQGYMP
YLNKYILDKVEEGAFVKEHFDELCWTLTAKKNYRA
DSNGNSMLSNQDAFRLWCLFNFLSEDKYPLIMVPD
EVEYLLKKVLSSMSLEVSLGELEELLAQEAQVAQT
TGGLSVWQFLELFNSGRCLRGVGRDTLSMAIHEVY
QELIQDVLKQGYLWKRGHLRRNWAERWFQLQPSCL
CYFGSEECKEKRGIIPLDAHCCVEVLPDRDGKRCM
FCVKTATRTYEMSASDTRQRQEWTAAIQMAIRLQA
EGKTSLHKDLKQKRREQREQR- Isotype
- IgG
- Antibody clone number
- 1F2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) λ/ι.
Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber TB, Schiffer M
The American journal of pathology 2013 Dec;183(6):1945-59
The American journal of pathology 2013 Dec;183(6):1945-59
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DEF6 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of DEF6 transfected lysate using anti-DEF6 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DEF6 monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to DEF6 on formalin-fixed paraffin-embedded human lymph node [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
