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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-109256 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ROC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HepG2, 293T, MCF-7, human colon cancer tissue, human stomach cancer tissue, mouse colon tissue, human placenta tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBX1 in HepG2 cell lysates. Samples were incubated with RBX1 polyclonal antibody (Product # PA5-109256), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RBX1 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RBX1 polyclonal antibody (Product # PA5-109256), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RBX1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RBX1 polyclonal antibody (Product # PA5-109256), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RBX1 in paraffin-embedded mouse colon tissue. Samples were incubated with RBX1 polyclonal antibody (Product # PA5-109256), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RBX1 in paraffin-embedded human stomach cancer tissue. Samples were incubated with RBX1 polyclonal antibody (Product # PA5-109256), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RBX1 in paraffin-embedded human placenta tissue. Samples were incubated with RBX1 polyclonal antibody (Product # PA5-109256), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of RBX1 in paraffin-embedded human colon cancer tissue. Samples were incubated with RBX1 polyclonal antibody (Product # PA5-109256), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of RBX1 in MCF-7 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with RBX1 polyclonal antibody (Product # PA5-109256) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
