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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- NB300-126 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-126, RRID:AB_2247910
- Product name
- Rabbit Polyclonal mGluR5/1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~125k monomer and the ~250k dimers of mGluR5 and mGluR1. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Autism-associated Shank3 mutations alter mGluR expression and mGluR-dependent but not NMDA receptor-dependent long-term depression.
Synaptic integration of newly generated neurons in rat dissociated hippocampal cultures.
The clock gene Per2 influences the glutamatergic system and modulates alcohol consumption.
Lee K, Vyas Y, Garner CC, Montgomery JM
Synapse (New York, N.Y.) 2019 Aug;73(8):e22097
Synapse (New York, N.Y.) 2019 Aug;73(8):e22097
Synaptic integration of newly generated neurons in rat dissociated hippocampal cultures.
Cheyne JE, Grant L, Butler-Munro C, Foote JW, Connor B, Montgomery JM
Molecular and cellular neurosciences 2011 Jul;47(3):203-14
Molecular and cellular neurosciences 2011 Jul;47(3):203-14
The clock gene Per2 influences the glutamatergic system and modulates alcohol consumption.
Spanagel R, Pendyala G, Abarca C, Zghoul T, Sanchis-Segura C, Magnone MC, Lascorz J, Depner M, Holzberg D, Soyka M, Schreiber S, Matsuda F, Lathrop M, Schumann G, Albrecht U
Nature medicine 2005 Jan;11(1):35-42
Nature medicine 2005 Jan;11(1):35-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: metabotropic Glutamate Receptor 5/1 Antibody [NB300-126] - 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR5/1a antibody recognizes both mGluR5 (Lane A) and mGluR1a (Lane B) labeling the ~125k monomer and the ~250k dimers for both mGluRs.
