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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- AGC-007-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- mGluR5 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.75 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat brainmembranes: - 1. Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:500). 2. Anti-mGluR5 (extracellular) Antibody , preincubated with mGluR5 (extracellular) Blocking Peptide (#BLP-GC007).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of mGluR5 in rat GH3 pituitary cells - Cell surface detection of mGluR5 in live intact rat GH3 pituitary cells using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:100), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of mGluR5 in rat GH3 pituitary cells - Cell surface detection of mGluR5 in live intact rat GH3 pituitary cells using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:100), followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of mGluR5 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in CA3 cells (arrows). Staining with mouse Anti-parvalbumin (green) revealed co-localization in pyramidal layer. DAPI counterstain was used to visualize nuclei of all cells (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of mGluR5 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat hippocampus sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in CA3 cells (arrows). Staining with mouse Anti-parvalbumin (green) revealed co-localization in pyramidal layer. DAPI counterstain was used to visualize nuclei of all cells (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of mGluR5 in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozenrat cerebellum sections using Anti-mGluR5 (extracellular) Antibody (#AGC-007), (1:50). mGluR5 (red) was detected in cerebellar Purkinje cells (vertical arrows) and in the molecular layer (horizontal arrows). Staining with mouse Anti-parvalbumin (green) revealed co-localization in Purkinje but not in the molecular layer. Little staining of mGluR5 was detected in the granule layer.DAPI counterstain is used to visualize nuclei of all cells (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of mGluR5 in live intact mouse BV-2 microglia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-mGluR5 (extracellular) Antibody (#AGC-007), (5 µg) + goat- Anti-rabbit-FITC.
