MA5-15518

antibody from Invitrogen Antibodies

Targeting: MUSK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15518

Provider

Invitrogen Antibodies

Product name

MUSK Monoclonal Antibody (10A4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15518 targets MUSK in IF and IHC applications and shows reactivity with Human samples.

Antibody clone number

10A4

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of MUSK was achieved by transfecting RD with MUSK specific siRNAs (Silencer® select Product # s224071 and s9085). Western blot analysis (Fig. a) was performed using whole cell extracts from the MUSK knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with MUSK Monoclonal Antibody (10A4) (Product # MA5-15518, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000) and detected using the iBright™ FL1500 Imaging System (Product # A44115). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MUSK. An uncharacterized band (*) was observed around 35 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using MUSK Monoclonal Antibody (10A4) (Product # MA5-15518) and a 180 kDa band corresponding to MUSK was observed in RD cell line and not observed in BeWo, SK-OV-3 and Raji cell lines. Whole cell extracts (40 µg lysate) of RD (Lane 1), BeWo (Lane 2), SK-O-V3 (Lane 3) and Raji (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). An uncharacterized band (*) was observed around 35 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HEK293 cells trasfected with extracellular MUSK (aa24-209) human IgG Fc using MUSK monoclonal antibody (Product # MA5-15518) (Green). Blue: DRAQ5 fluorescent DNA dye.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HEK293 cells trasfected with extracellular MUSK (aa24-209) human IgG Fc using MUSK monoclonal antibody (Product # MA5-15518) (Green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung cancer (A), muscles (B) and breast cancer (C) using MUSK monoclonal antibody (Product # MA5-15518) followed with DAB staining.