Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-15518 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MUSK Monoclonal Antibody (10A4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 10A4
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of MUSK was achieved by transfecting RD with MUSK specific siRNAs (Silencer® select Product # s224071 and s9085). Western blot analysis (Fig. a) was performed using whole cell extracts from the MUSK knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with MUSK Monoclonal Antibody (10A4) (Product # MA5-15518, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000) and detected using the iBright™ FL1500 Imaging System (Product # A44115). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MUSK. An uncharacterized band (*) was observed around 35 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using MUSK Monoclonal Antibody (10A4) (Product # MA5-15518) and a 180 kDa band corresponding to MUSK was observed in RD cell line and not observed in BeWo, SK-OV-3 and Raji cell lines. Whole cell extracts (40 µg lysate) of RD (Lane 1), BeWo (Lane 2), SK-O-V3 (Lane 3) and Raji (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). An uncharacterized band (*) was observed around 35 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HEK293 cells trasfected with extracellular MUSK (aa24-209) human IgG Fc using MUSK monoclonal antibody (Product # MA5-15518) (Green). Blue: DRAQ5 fluorescent DNA dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HEK293 cells trasfected with extracellular MUSK (aa24-209) human IgG Fc using MUSK monoclonal antibody (Product # MA5-15518) (Green). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer (A), muscles (B) and breast cancer (C) using MUSK monoclonal antibody (Product # MA5-15518) followed with DAB staining.