Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-75311 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TSPAN8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity.
Kumar B, Adebayo AK, Prasad M, Capitano ML, Wang R, Bhat-Nakshatri P, Anjanappa M, Simpson E, Chen D, Liu Y, Schilder JM, Colter AB, Maguire C, Temm CJ, Sandusky G, Doud EH, Wijeratne AB, Mosley AL, Broxmeyer HE, Nakshatri H
Science advances 2022 Jan 14;8(2):eabh3375
Science advances 2022 Jan 14;8(2):eabh3375
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TSPAN8 in Lane 1: Hct116 whole cell lysate, Lane 2: SGC7901 whole cell lysate, Lane 3: DLD whole cell lysate, Lane 4: the Kidney tissue lysate of mouse, Lane 5: the Intestines tissue lysate of rat, Lane 6: BV2 whole cell lysate. Samples were incubated with TSPAN8 polyclonal antibody (Product # PA5-75311) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TSPAN8 in Lane 1: CT26 whole cell lysate (40 µg), Lane 2: PC12 whole cell lysate (40 µg), Lane 3: MCF-7 whole cell lysate (40 µg), Lane 4: HepG2 whole cell lysate (40 µg), Lane 5: HCT116 whole cell lysate (40 µg). Samples were incubated with TSPAN8 polyclonal antibody (Product # PA5-75311) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TSPAN8 in paraffin-embedded human tonsil carcinoma tissue. Samples were incubated with TSPAN8 polyclonal antibody (Product # PA5-75311) at a dilution of 1:50.