Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM09069PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09069PU-S, RRID:AB_1876007
- Product name
- anti Transglutaminase-4 (TGM4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant human TGM4 (421-565 aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT1H1
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of LNCaP (25 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TGM4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Cell lysates of LNCaP (25ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TGM4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: Paraffin embedded sections of Human breast cancer tissue were incubated with anti-Human TGM4 antibody (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1 M Sodium Citrate buffer and detected using Diaminobenzidine (DAB).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human breast cancer tissue were incubated with anti-human TGM4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
