Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF6897 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse PAK3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human PAK3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PAK1, rhPAK1B, rhPAK2, rhPAK4, rhPAK6, and rhPAK7 is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
O75914- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PAK3 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human PAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6897) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PAK3 in Human Brain. PAK3 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human PAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6897) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PAK3 in Mouse Embryo. PAK3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human PAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6897) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neuronal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
