Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- APR-021-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2Y1 Receptor (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates: - 1-3. Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:200).4-6. Anti-P2Y1 Receptor (extracellular) Antibody , preincubated with P2Y1 Receptor (extracellular) Blocking Peptide (#BLP-PR021).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of P2RY1 in rat PC12 cells - Cell surface detection of P2RY1 in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Merge of extracellular staining with live view of the cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of P2RY1 in rat PC12 cells - Cell surface detection of P2RY1 in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Merge of extracellular staining with live view of the cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of P2RY1 in mouse hippocampus - Immunohistochemical staining of mouse hippocampal CA3 region using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021). A. P2RY1 staining (red) appears in the pyramidal layer (arrows). B. Nuclei staining using DAPI as the counterstain (blue).C. Merged images of panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of P2RY1 in Jurkat (human acute T cell leukemia) living cells: - (black) Unstained cells. (red) Cells stained + Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (5 μg/1x106cells).