25684-1-AP

antibody from Invitrogen Antibodies

Targeting: ECPAS ECM29, FLJ22036, KIAA0368

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

25684-1-AP

Provider

Invitrogen Antibodies

Product name

KIAA0368 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EECNLWTEV WQENVPGSFG GIRLYLQELI TITQKALQSQ SWKMKAQGAI AMASIAKQTS SLVPPYLGMI LTALLQGLAG RTWAGKEELL KAIACVVTAC SAELEKSVPN QPSTNEILQA VLKECSKENV KYKIVAISCA ADILKATKED RFQEFSNIVI PLIKKNSLES SGVRTTKNEE ENEKEKELQL EYLLGAFESL GKAWPRNAET QRCYRQELCK LMCERLKLST WKVQLGVLQS MNAFFQGLML LEEEHADPEA LAEILLETCK SITYSLENKT YSSVRTEALS VIELLLKKLE ESKQWECLTS ECRVLLIESL ATMEPDSRPE LQEKAALLKK TLENLE (1501-1845 aa encoded by BC021127)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.32 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 25684-1-AP ( KIAA0368 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 25684-1-AP (KIAA0368 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HEK-293 cells using 25684-1-AP (KIAA0368 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HEK-293 cells using 25684-1-AP (KIAA0368 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 25684-1-AP ( KIAA0368 antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 25684-1-AP ( KIAA0368 antibody) at dilution of 1:200 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-KIAA0368 (IP:25684-1-AP, 4ug; Detection:25684-1-AP 1:600) with HEK-293 cells lysate 2600ug.