Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-28693 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28693, RRID:AB_1913941
- Product name
- Rabbit Polyclonal DHX57 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: DHX57 Antibody [NBP1-28693] - Detection of Human DHX57 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX57 antibody A302-299A used for IP at 10 mcg/mg lysate. DHX57 was also immunoprecipitated by rabbit anti-DHX57 antibody A302-300A, which recognizes a downstream epitope. For blotting immunoprecipitated DHX57, A302-300A was used at 0.4 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 30 seconds.