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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA3-006 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- IHC (P) analysis shows positive staining of GPR18 in human enteric ganglion tissue.
- Concentration
- Conc. Not Determined
Submitted references Resolvin D2 uses multiple Ca(2+) -dependent signaling pathways to stimulate mucin secretion in rat and human conjunctival goblet cells.
Botten N, Hodges RR, Bair J, Utheim TP, Serhan CN, Yang M, Dartt DA
Journal of cellular physiology 2022 Oct;237(10):3816-3833
Journal of cellular physiology 2022 Oct;237(10):3816-3833
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPR18 was performed by loading equal amounts of wheat germ lectin agarose bead enriched GPR receptor fractions from GPR18 or GPR3 transfected HEK293 lysates and 10 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) for 1 hour at room temperature. GPR18 was detected at ~70 kDa using a GPR18 polyclonal antibody (Product # PA3-006) at a dilution of 1:1000 in StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). Images were acquired on a Thermo Scientific myECL Imager (Product # 62236).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GPR18 was performed on human enteric ganglion tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a GPR18 polyclonal antibody (Product # PA3-006) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washed, and detection was performed using an ABC kit composed of biotinylated goat anti-rabbit IgG, peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole (AEC) substrate in acetate buffer. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting.
