Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS3462 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS3462, RRID:AB_1661928
- Product name
- Nephrin (H892) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 861-910 of Human Nephrin.
- Description
- Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of Nephrin (H892) pAb at 1:1000 dilution Lane1:The muscle tissue lysate of Rat(47ug) Lane2:HEK293T whole cell lysate(41ug) Lane3:Jurkat whole cell lysate(41ug) Lane4:H9C2 whole cell lysate(60ug)