Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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- Product number
- AF3159 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Nephrin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Nephrin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Nephrin is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_062332
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A KDM6A-KLF10 reinforcing feedback mechanism aggravates diabetic podocyte dysfunction.
Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin.
Accelerated Glomerular Cell Senescence in Experimental Lupus Nephritis.
Endoplasmic reticulum stress inhibition attenuates hypertensive chronic kidney disease through reduction in proteinuria.
Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy.
Natriuretic peptide receptor guanylyl cyclase-A pathway counteracts glomerular injury evoked by aldosterone through p38 mitogen-activated protein kinase inhibition.
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice.
Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy.
Smad3 deficiency protects mice from obesity-induced podocyte injury that precedes insulin resistance.
TGF-β-activated kinase 1 is crucial in podocyte differentiation and glomerular capillary formation.
Morphine induces albuminuria by compromising podocyte integrity.
Glomerular endothelial surface layer acts as a barrier against albumin filtration.
Divergent roles of Smad3 and PI3-kinase in murine adriamycin nephropathy indicate distinct mechanisms of proteinuria and fibrogenesis.
Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
Lin CL, Hsu YC, Huang YT, Shih YH, Wang CJ, Chiang WC, Chang PJ
EMBO molecular medicine 2019 May;11(5)
EMBO molecular medicine 2019 May;11(5)
Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin.
Li F, Mao X, Zhuang Q, Zhao Z, Zhang Z, Wu H
Cell death & disease 2019 Mar 11;10(3):241
Cell death & disease 2019 Mar 11;10(3):241
Accelerated Glomerular Cell Senescence in Experimental Lupus Nephritis.
Yang C, Xue J, An N, Huang XJ, Wu ZH, Ye L, Li ZH, Wang SJ, Pan QJ, Liang D, Liu HF
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 28;24:6882-6891
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 28;24:6882-6891
Endoplasmic reticulum stress inhibition attenuates hypertensive chronic kidney disease through reduction in proteinuria.
Mohammed-Ali Z, Lu C, Marway MK, Carlisle RE, Ask K, Lukic D, Krepinsky JC, Dickhout JG
Scientific reports 2017 Feb 2;7:41572
Scientific reports 2017 Feb 2;7:41572
Deficient Insulin-mediated Upregulation of the Equilibrative Nucleoside Transporter 2 Contributes to Chronically Increased Adenosine in Diabetic Glomerulopathy.
Alarcón S, Garrido W, Cappelli C, Suárez R, Oyarzún C, Quezada C, San Martín R
Scientific reports 2017 Aug 25;7(1):9439
Scientific reports 2017 Aug 25;7(1):9439
Natriuretic peptide receptor guanylyl cyclase-A pathway counteracts glomerular injury evoked by aldosterone through p38 mitogen-activated protein kinase inhibition.
Kato Y, Mori K, Kasahara M, Osaki K, Ishii A, Mori KP, Toda N, Ohno S, Kuwabara T, Tokudome T, Kishimoto I, Saleem MA, Matsusaka T, Nakao K, Mukoyama M, Yanagita M, Yokoi H
Scientific reports 2017 Apr 21;7:46624
Scientific reports 2017 Apr 21;7:46624
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice.
Sung SJ, Ge Y, Dai C, Wang H, Fu SM, Sharma R, Hahn YS, Yu J, Le TH, Okusa MD, Bolton WK, Lawler JR
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2589-2601
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2589-2601
Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy.
Fujino T, Hasebe N
BMC nephrology 2016 Nov 17;17(1):179
BMC nephrology 2016 Nov 17;17(1):179
Smad3 deficiency protects mice from obesity-induced podocyte injury that precedes insulin resistance.
Sun YB, Qu X, Howard V, Dai L, Jiang X, Ren Y, Fu P, Puelles VG, Nikolic-Paterson DJ, Caruana G, Bertram JF, Sleeman MW, Li J
Kidney international 2015 Aug;88(2):286-98
Kidney international 2015 Aug;88(2):286-98
TGF-β-activated kinase 1 is crucial in podocyte differentiation and glomerular capillary formation.
Kim SI, Lee SY, Wang Z, Ding Y, Haque N, Zhang J, Zhou J, Choi ME
Journal of the American Society of Nephrology : JASN 2014 Sep;25(9):1966-78
Journal of the American Society of Nephrology : JASN 2014 Sep;25(9):1966-78
Morphine induces albuminuria by compromising podocyte integrity.
Lan X, Rai P, Chandel N, Cheng K, Lederman R, Saleem MA, Mathieson PW, Husain M, Crosson JT, Gupta K, Malhotra A, Singhal PC
PloS one 2013;8(3):e55748
PloS one 2013;8(3):e55748
Glomerular endothelial surface layer acts as a barrier against albumin filtration.
Dane MJ, van den Berg BM, Avramut MC, Faas FG, van der Vlag J, Rops AL, Ravelli RB, Koster BJ, van Zonneveld AJ, Vink H, Rabelink TJ
The American journal of pathology 2013 May;182(5):1532-40
The American journal of pathology 2013 May;182(5):1532-40
Divergent roles of Smad3 and PI3-kinase in murine adriamycin nephropathy indicate distinct mechanisms of proteinuria and fibrogenesis.
Finer G, Schnaper HW, Kanwar YS, Liang X, Lin HY, Hayashida T
Kidney international 2012 Sep;82(5):525-36
Kidney international 2012 Sep;82(5):525-36
Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
Pierchala BA, Muñoz MR, Tsui CC
Kidney international 2010 Nov;78(9):868-82
Kidney international 2010 Nov;78(9):868-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nephrin in Mouse Kidney. Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Nephrin in Mouse Kidney. Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.