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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-91907 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nephrin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: Mouse kidney, mouse brain, rat brain; Cellular Location: Cell membrane, Single-pass type I membrane protein Immunogen sequence: LGDSGLADKG TQLPITTPGL HQPSGEPEDQ LPTEPPSGPS GLPLLPVLFA LGGLLLLSNA SCVGGVLWQR RLRRLAEGIS EKTEAGSEED RVRNEYEESQ WTGERDTQSS TVSTTEAEPY YRSLRDFSPQ LPPTQEEVSY SRGFTGEDED MAFPGHLYDE VERTYPPSGA WGPLYDEVQM GPWDLHWPED TYQDPRGIYD QVAGDLDTLE PDSLPFELRG HLV
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Twist1 in podocytes ameliorates podocyte injury and proteinuria by limiting CCL2-dependent macrophage infiltration.
Ren J, Xu Y, Lu X, Wang L, Ide S, Hall G, Souma T, Privratsky JR, Spurney RF, Crowley SD
JCI insight 2021 Aug 9;6(15)
JCI insight 2021 Aug 9;6(15)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of mouse kidney, using NPHS1 Polyclonal antibody (Product # PA5-91907) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Nephrin in extracts of mouse kidney using Nephrin Polyclonal Antibody (Product # PA5-91907) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Nephrin in extracts of various cell lines using Nephrin Polyclonal Antibody (Product # PA5-91907) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nephrin in paraffin-embedded mouse kidney using Nephrin Polyclonal Antibody (Product # PA5-91907) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of Nephrin was performed in mouse kidney tissue using Nephrin Polyclonal Antibody (Product # PA5-91907) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Twist1 in podocytes limits albuminuria and podocyte injury induced by NTS in mice. ( A ) Urinary albumin concentration in WT and Twist1-PKO mice on day 9 vehicle or NTS ( n = 4-9, Kruskal-Wallis test). ( B ) Representative SDS-PAGE showing the urine proteins in cohorts of mice. ( C ) Representative images of kidney sections from WT and Twist1-PKO mice on day 9 vehicle or NTS. Red arrowheads indicate glomerulosclerosis; red arrows indicate tubular casts. Scale bar: 40 mum. ( D ) Kidney glomerulosclerosis scores in groups ( n = 4-9, Kruskal-Wallis test). ( E ) Renal mRNA expression of podocin, nephrin, and podocalyxin in WT and Twist1-PKO mice after NTS injection (7-9, t test). ( F ) Representative Western blots for nephrin and podocin proteins in glomeruli isolated from WT and Twist1-PKO mice on day 4 NTS. ( G ) Semiquantitative determination of nephrin and podocin protein levels from blots in F ( n = 7-9, t test). ( H ) Representative immunostaining for WT1 protein in the groups and electron microscopy showing the glomerular filtration barrier in the groups. ( I ) Quantitative determination of WT1-positive cells in glomeruli in different groups ( n = 3-6, Student-Newman-Keuls test). Data represent mean +- SEM. All t tests were 2 tailed. * P < 0.05, # P < 0.05. Red arrowheads indicate foot processes. Scale bar: 40 mum (upper), 1 mum (lower). veh, vehicle; NTS, nephrotoxic serum; EM, electron microscopy; Twist1-PKO, Pod-Cre Twist1 fl/fl .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Twist1 in podocytes mitigates albuminuria and podocyte injury induced by ADR in mice. ( A ) Urinary albumin concentration in WT and Twist1-PKO mice on day 1 (left) and day 4 (right) ADR ( n = 4-6, t test). ( B ) Renal mRNA expression of podocin, nephrin, and podocalyxin in WT and Twist1-PKO mice after ADR injection ( n = 4-5, t test). ( C ) Western blot for podocin, nephrin, and WT1 protein in glomeruli isolated from WT and Twist1-PKO mice after ADR injection. ( D ) Semiquantitative determination of podocin, nephrin, and WT1 protein from blots in C (6-7, t test). ( E ) EM shows glomerular filtration barrier in different groups after ADR exposure. Red arrowheads indicate foot processes. Scale bar: 1 mum. Data represent mean +- SEM. All t tests were 2 tailed. * P < 0.05. ADR, adriamycin; EM, electron microscopy; Twist1-PKO, Pod-Cre Twist1 fl/fl .
