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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB5170 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CLEC3B/Tetranectin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CLEC3B/Tetranectin in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P05452- Isotype
- IgG
- Antibody clone number
- 805035
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CLEC3B/Tetranectin by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC3B/Tetranectin at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CLEC3B/Tetranectin by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. Specific bands were detected for CLEC3B/Tetranectin at approximately 32 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human CLEC3B/Tetranectin Monoclonal Antibody (Catalog # MAB5170). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
