Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-79399 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSD11B2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Metabolomics analysis of follicular fluid coupled with oocyte aspiration reveals importance of glucocorticoids in primate periovulatory follicle competency.
Ravisankar S, Hanna CB, Brooks KE, Murphy MJ, Redmayne N, Ryu J, Kinchen JM, Chavez SL, Hennebold JD
Scientific reports 2021 Mar 22;11(1):6506
Scientific reports 2021 Mar 22;11(1):6506
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSD11B2 in Lane 1: rat kidney tissue lysate, Lane 2: human placenta tissue lysate using 50 µg per well. Sample was incubated with HSD11B2 (Product # PA5-79399) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSD11B2 in Lane 1: rat kidney tissue lysate, Lane 2: human placenta tissue lysate using 50 µg per well. Sample was incubated with HSD11B2 (Product # PA5-79399) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSD11B2 in, Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates, Lane 3: human placenta tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with HSD11B2 Polyclonal Antibody (Product # PA5-79399) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for HSD11B2 at approximately 43 kDa. The expected band size for HSD11B2 is at 44 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSD11B2 on paraffin-embedded rat pancreas tissue. Sample was incubated with HSD11B2 polyclonal antibody (Product# PA5-79399).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSD11B2 on paraffin-embedded mouse pancreas tissue. Sample was incubated with HSD11B2 polyclonal antibody (Product# PA5-79399).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSD11B2 on paraffin-embedded human placenta tissue. Sample was incubated with HSD11B2 polyclonal antibody (Product# PA5-79399).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Glucocorticoids and the enzymes that metabolize glucocorticoids are present in the rhesus macaque periovulatory follicle. ( a ) LC-MS/MS analysis of cortisol and cortisone concentrations in the FF obtained from rhesus macaque follicles between pre- (0 h) and 36 h post-hCG administration. A statistically significant increase in cortisol (* p = 0.0137) and decrease in cortisone (* p = 0.0110), with a corresponding increase in the cortisol to cortisone ratio (* p = 0.0256), was observed following hCG injection. ( b ) IHC of HSD11B1 and ( c ) HSD11B2 immunolocalization in the rhesus macaque periovulatory follicle pre- (0 h) as well as 12 h, 24 h and 36 h post-hCG administration. The images shown are representative of N = 4 ovaries obtained from separate animals undergoing a COv protocol at each of the times indicated. Note the overall increase in HSD11B1, as well as a concomitant decrease in HSD11B2 expression, in the ovarian follicle prior to hCG administration and with increasing time after the ovulatory stimulus. O = oocyte, C = cumulus cells, A = antrum, T = theca cells, M = mural granulosa cells and NC = negative control.