Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF634 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse AgRP/ART Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse AgRP in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human AgRP is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P56473
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references High-fat diet accelerates extreme obesity with hyperphagia in female heterozygous Mecp2-null mice.
A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism.
QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior.
MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
Fukuhara S, Nakajima H, Sugimoto S, Kodo K, Shigehara K, Morimoto H, Tsuma Y, Moroto M, Mori J, Kosaka K, Morimoto M, Hosoi H
PloS one 2019;14(1):e0210184
PloS one 2019;14(1):e0210184
A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism.
Ou Z, Ma Y, Sun Y, Zheng G, Wang S, Xing R, Chen X, Han Y, Wang J, Lu QR, Zhao TJ, Chen Y
Cell reports 2019 Mar 12;26(11):2984-2997.e4
Cell reports 2019 Mar 12;26(11):2984-2997.e4
QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior.
Okamoto K, Yamasaki M, Takao K, Soya S, Iwasaki M, Sasaki K, Magoori K, Sakakibara I, Miyakawa T, Mieda M, Watanabe M, Sakai J, Yanagisawa M, Sakurai T
PloS one 2016;11(11):e0164716
PloS one 2016;11(11):e0164716
MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
Cortes-Campos C, Elizondo R, Carril C, MartÃnez F, Boric K, Nualart F, Garcia-Robles MA
PloS one 2013;8(4):e62532
PloS one 2013;8(4):e62532
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- AgRP/ART in Mouse Brain. AgRP/ART was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Goat Anti-Mouse AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF634) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.