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LearnH00084063-M01
antibody from Abnova Corporation
Targeting: KIRREL2
DKFZp564A1164, FILTRIN, MGC15718, NEPH3, NLG1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00084063-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00084063-M01, RRID:AB_426090
- Product name
- KIRREL2 monoclonal antibody (M01), clone 2B9-1D3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KIRREL2.
- Antigen sequence
MLRMRVPALLVLLFCFRGRAGPSPHLLQQPEDLVV
LLGEGGAQASLGRRASASFSEQKNLMRIPGSSDGS
SSRGPEEEETGSREDRGPIVHTDHSDLVLEEEGTL
ETKDPTNGYYKVRGVSVSLSLGEAPGGGLFLPPPS
PLGPPGTPTFYDFNPHLGMVPPCRLYRARAGYLTT
PHPRAFTSYIKPTSFGPPDLAPGTPPFPYAAFPTP
SHPRLQTHV- Isotype
- IgG
- Antibody clone number
- 2B9-1D3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KIRREL2 monoclonal antibody (M01), clone 2B9-1D3 Western Blot analysis of KIRREL2 expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KIRREL2 expression in transfected 293T cell line by KIRREL2 monoclonal antibody (M01), clone 2B9-1D3.Lane 1: KIRREL2 transfected lysate (Predicted MW: 23.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KIRREL2 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of KIRREL2 transfected lysate using anti-KIRREL2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with KIRREL2 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
