Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-28491 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIMD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, mouse brain. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (81%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LIMD1 Polyclonal Antibody (Product # PA5-28491). Sample (30 µg whole cell lysate). A: 293T. 12% SDS PAGE. LIMD1 Polyclonal Antibody (Product # PA5-28491) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LIMD1 Polyclonal Antibody (Product # PA5-28491). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. LIMD1 Polyclonal Antibody (Product # PA5-28491) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LIMD1 Polyclonal Antibody detects LIMD1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LIMD1 protein stained by LIMD1 Polyclonal Antibody (Product # PA5-28491) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of LIMD1 was performed in paraffin-embedded mouse brain tissue using LIMD1 Polyclonal Antibody (Product # PA5-28491) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using LIMD1 (Product # PA5-28491) antibody at 1:300 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.