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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 24550-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMOT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin. This antibody recognizes both p130 and p80.
- Concentration
- 0.57 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of AMOT antibody (24550-1-AP, 1:6000) with si-Control and si-AMOT transfected HEK293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB results of 24550-1-AP (1:1000) with over-expressed AMOT by Customer.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 24550-1-AP (AMOT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 24550-1-AP (AMOT antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 24550-1-AP (AMOT antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-AMOT (IP:24550-1-AP, 4ug; Detection:24550-1-AP 1:300) with HEK-293 cells lysate 2500ug.
