GTX104703

antibody from GeneTex

Targeting: HTR1A 5-HT1A, ADRB2RL1, ADRBRL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX104703

Provider

GeneTex

Proper citation

GeneTex Cat#GTX104703, RRID:AB_1241307

Product name

5-HT1A receptor antibody [N3C1], Internal

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

LRRK2 G2019S Induces Anxiety/Depression-like Behavior before the Onset of Motor Dysfunction with 5-HT(1A) Receptor Upregulation in Mice.

Lim J, Bang Y, Choi JH, Han A, Kwon MS, Liu KH, Choi HJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 14;38(7):1611-1621

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Deletion of mouse FXR gene disturbs multiple neurotransmitter systems and alters neurobehavior.

Huang F, Wang T, Lan Y, Yang L, Pan W, Zhu Y, Lv B, Wei Y, Shi H, Wu H, Zhang B, Wang J, Duan X, Hu Z, Wu X
Frontiers in behavioral neuroscience 2015;9:70

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Applications of yeast-based signaling sensor for characterization of antagonist and analysis of site-directed mutants of the human serotonin 1A receptor.

Nakamura Y, Ishii J, Kondo A
Biotechnology and bioengineering 2015 Sep;112(9):1906-15

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Prenatal lipopolysaccharide exposure increases anxiety-like behaviors and enhances stress-induced corticosterone responses in adult rats.

Lin YL, Lin SY, Wang S
Brain, behavior, and immunity 2012 Mar;26(3):459-68

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) B: Raji 10% SDS PAGE GTX104703 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5HT1A receptor antibody [N3C1], Internal detects 5HT1A receptor protein by western blot analysis.A. 20 ?g D-hippocampus C9 lysate/extract10% SDS-PAGE5HT1A receptor antibody [N3C1], Internal (GTX104703) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5HT1A Receptor antibody [N3C1], Internal detects 5HT1A Receptor protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. 5HT1A Receptor antibody [N3C1], Internal (GTX104703) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5HT1A Receptor antibody [N3C1], Internal detects 5HT1A Receptor protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. 5HT1A Receptor antibody [N3C1], Internal (GTX104703) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

5-HT1A receptor antibody [N3C1], Internal detects 5-HT1A receptor protein by immunohistochemical analysis. Samples: Frozen Sectioned adult mouse hippocampus.Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal (GTX104703) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).