PA5-99483

antibody from Invitrogen Antibodies

Targeting: HTR1A 5-HT1A, ADRB2RL1, ADRBRL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-99483

Provider

Invitrogen Antibodies

Product name

HTR1A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HTR1A in 293 cell lysate (left lane: treated with blocking peptide). Samples were incubated with HTR1A polyclonal antibody (Product # PA5-99483).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HTR1A in 293 cell lysate (left lane: treated with blocking peptide). Samples were incubated with HTR1A polyclonal antibody (Product # PA5-99483).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HTR1A in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and HTR1A polyclonal antibody (Product # PA5-99483) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).