H00065057-M02

antibody from Novus Biologicals

Targeting: ACD Pip1, Ptop, Tint1, Tpp1

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

H00065057-M02

Provider

Novus Biologicals

Proper citation

Novus Cat#H00065057-M02, RRID:AB_536552

Product name

Mouse Monoclonal ACD Antibody

Antibody type

Monoclonal

Description

IgG purified. ACD - adrenocortical dysplasia homolog (mouse)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

An N-terminal Flag-tag impairs TPP1 regulation of telomerase function.

Sandhu R, Wei D, Sharma M, Xu L
Biochemical and biophysical research communications 2019 Apr 30;512(2):230-235

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Human cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion.

Zanini IM, Soneson C, Lorenzi LE, Azzalin CM
Journal of cell science 2017 Feb 15;130(4):767-778

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The Shelterin TIN2 Subunit Mediates Recruitment of Telomerase to Telomeres.

Frank AK, Tran DC, Qu RW, Stohr BA, Segal DJ, Xu L
PLoS genetics 2015 Jul;11(7):e1005410

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Hoyeraal-Hreidarsson syndrome caused by a germline mutation in the TEL patch of the telomere protein TPP1.

Kocak H, Ballew BJ, Bisht K, Eggebeen R, Hicks BD, Suman S, O'Neil A, Giri N, NCI DCEG Cancer Genomics Research Laboratory, NCI DCEG Cancer Sequencing Working Group, Maillard I, Alter BP, Keegan CE, Nandakumar J, Savage SA
Genes & development 2014 Oct 1;28(19):2090-102

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The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity.

Nandakumar J, Bell CF, Weidenfeld I, Zaug AJ, Leinwand LA, Cech TR
Nature 2012 Dec 13;492(7428):285-9

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A ssDNA Aptamer That Blocks the Function of the Anti-FLAG M2 Antibody.

Lakamp AS, Ouellette MM
Journal of nucleic acids 2011;2011:720798

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Characterization of the DNA binding specificity of Shelterin complexes.

Choi KH, Farrell AS, Lakamp AS, Ouellette MM
Nucleic acids research 2011 Nov;39(21):9206-23

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TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo.

Abreu E, Aritonovska E, Reichenbach P, Cristofari G, Culp B, Terns RM, Lingner J, Terns MP
Molecular and cellular biology 2010 Jun;30(12):2971-82

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: ACD Antibody (1D8-1B6) [H00065057-M02] - Detection limit for recombinant GST tagged ACD is 0.3 ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of monoclonal antibody to ACD on HeLa cell. Antibody concentration 10 ug/ml.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of ACD transfected lysate using anti-ACD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACD MaxPab rabbit polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of monoclonal antibody to ACD on formalin-fixed paraffin-embedded human heart tissue. Antibody concentration 2 ug/ml.